2型单纯疱疹病毒在低病毒滴度时呈现非典型的解链温度曲线。
Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers.
作者信息
LeBlanc Jason J, Campbell Sarah, Pettipas Janice, Hatchette Todd F, Davidson Ross J
机构信息
Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada.
出版信息
J Clin Microbiol. 2008 Aug;46(8):2786-9. doi: 10.1128/JCM.02177-07. Epub 2008 Jun 4.
Abstract
Real-time PCR is a powerful tool for the detection and typing of herpes simplex virus (HSV). HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (T(m)s) of the hybridization probes. The efficacy of T(m) profiling with low DNA concentrations was evaluated in this study.
摘要
实时聚合酶链反应（PCR）是检测和分型单纯疱疹病毒（HSV）的有力工具。通过利用杂交探针熔解温度（T(m)）的差异，可以区分HSV-1型和HSV-2型。本研究评估了低DNA浓度下T(m)分析的效果。
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