尿嘧啶-DNA糖基化酶（UNG）会影响单纯疱疹病毒（HSV）杂交探针的解链温度（T(m)）。
Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.
作者信息
Leblanc Jason J, Pettipas Janice, Campbell Sarah J, Davidson Ross J, Hatchette Todd F
机构信息
Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada.
出版信息
J Virol Methods. 2008 Jul;151(1):158-60. doi: 10.1016/j.jviromet.2008.03.024. Epub 2008 May 12.
Abstract
Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added.
摘要
在进行聚合酶链反应（PCR）扩增之前，可以加入尿嘧啶-DNA糖基化酶（UNG）以防止扩增子污染。利用单纯疱疹病毒（HSV）杂交探针的熔解温度（T(m)）分析来证明，当加入热稳定或热不稳定的UNG时，T(m)值会呈浓度依赖性降低。
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