Ebata Kevin T, Yeh Jonathan R, Zhang Xiangfan, Nagano Makoto C
Department of Obstetrics and Gynecology, McGill University, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec H3A 1A1, Canada.
Dis Markers. 2008;24(4-5):267-76. doi: 10.1155/2008/536020.
Spermatogonial stem cells (SSCs) are defined by their ability to both self-renew and produce differentiated germ cells that will develop into functional spermatozoa. Because of this ability, SSCs can reestablish spermatogenesis after testicular damage caused by cytotoxic agents or after transplantation into an infertile recipient. Therefore, SSCs are an important target cell for restoring male fertility, particularly for cancer patients who have to undergo sterilizing cancer therapies. In the mouse, the identification of SSC markers allows for the isolation of a highly enriched population of stem cells. This enriched stem cell population can be expanded in culture for an indefinite period of time, cryopreserved, and transplanted into infertile recipients to restore fertility. Thus, the identification of markers and the establishment of a long-term culture system for human SSCs will be crucial for realizing the potential of these cells in a clinical setting. In this article, we focus on the markers that have been identified for mouse SSCs and discuss how human SSC markers may be used in the restoration of fertility.
精原干细胞（SSCs）的定义是它们既能自我更新，又能产生分化的生殖细胞，这些生殖细胞将发育成功能性精子。由于这种能力，精原干细胞可以在细胞毒性药物引起的睾丸损伤后或移植到不育受体后重新建立精子发生。因此，精原干细胞是恢复男性生育力的重要靶细胞，特别是对于那些必须接受绝育癌症治疗的癌症患者。在小鼠中，精原干细胞标志物的鉴定使得能够分离出高度富集的干细胞群体。这种富集的干细胞群体可以在培养中无限期扩增、冷冻保存，并移植到不育受体中以恢复生育力。因此，人类精原干细胞标志物的鉴定以及长期培养系统的建立对于在临床环境中实现这些细胞的潜力至关重要。在本文中，我们重点关注已鉴定出的小鼠精原干细胞标志物，并讨论人类精原干细胞标志物如何用于恢复生育力。
