Dixon R Brent, Sampson Jason S, Hawkridge Adam M, Muddiman David C
W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
Anal Chem. 2008 Jul 1;80(13):5266-71. doi: 10.1021/ac800289f. Epub 2008 Jun 5.
The use of aerodynamic devices in ambient ionization source development has become increasingly prevalent in the field of mass spectrometry. In this study, an air ejector has been constructed from inexpensive, commercially available components to incorporate an electrospray ionization emitter within the exhaust jet of the device. This novel aerodynamic device, herein termed remote analyte sampling, transport, and ionization relay (RASTIR) was used to remotely sample neutral species in the ambient and entrain them into an electrospray plume where they were subsequently ionized and detected using a linear ion trap Fourier transform mass spectrometer. Two sets of experiments were performed in the ambient environment to demonstrate the device's utility. The first involved the remote (approximately 1 ft) vacuum collection of pure sample particulates (i.e., dry powder) from a glass slide, entrainment and ionization at the ESI emitter, and mass spectrometric detection. The second experiment involved the capture (vacuum collection) of matrix-assisted laser desorbed proteins followed by entrainment in the ESI emitter plume, multiple charging, and mass spectrometric detection. This approach is in principle a RASTIR-assisted matrix-assisted laser desorption electrospray ionization source (Sampson, J. S.; Hawkridge, A. M.; Muddiman, D. C. J. Am. Soc. Mass Spectrom. 2006, 17, 1712-1716; Rapid Commun. Mass Spectrom. 2007, 21, 1150-1154.). A detailed description of the device construction, operational parameters, and preliminary small molecule and protein data are presented.
在环境电离源开发中，气动装置的应用在质谱领域已变得越来越普遍。在本研究中，已用价格低廉的商用组件构建了一个空气喷射器，以便将电喷雾电离发射器纳入该装置的排气流中。这种新型气动装置，在此称为远程分析物采样、传输和电离中继器（RASTIR），用于在环境中远程采样中性物质，并将它们夹带至电喷雾羽流中，随后在那里它们被电离，并使用线性离子阱傅里叶变换质谱仪进行检测。在环境中进行了两组实验以证明该装置的效用。第一个实验涉及从载玻片远程（约1英尺）真空收集纯样品颗粒（即干粉），在电喷雾电离发射器处夹带和电离，以及质谱检测。第二个实验涉及捕获（真空收集）基质辅助激光解吸的蛋白质，随后将其夹带至电喷雾电离发射器羽流中，多次充电，以及质谱检测。这种方法原则上是一种RASTIR辅助的基质辅助激光解吸电喷雾电离源（桑普森，J.S.；霍克里奇，A.M.；穆迪曼，D.C.《美国质谱学会杂志》2006年，17卷，1712 - 1716页；《快速质谱通讯》2007年，21卷，1150 - 1154页）。本文给出了该装置构造、操作参数以及小分子和蛋白质初步数据的详细描述。
