Hillhouse E W, Jones M T
J Endocrinol. 1976 Oct;71(1):21-30. doi: 10.1677/joe.0.0710021.
The rat hypothalamus in vitro preparation was used to investigate the effect of bilateral adrenalectomy, with and without replacement therapy, on the release of corticotrophin-releasing factor(CRF). Corticotrophin-releasing factor was estimated using 48 h basal hypothalamic lesioned assay rats and corticosterone production of excised adrenals was used as the end point. Bilateral adrenalectomy resulted in depletion of hypothalamic CRF content within the first 2 h after the operation but this effect was prevented by replacement therapy with corticosterone. Thereafter, the hypothalamic CRF content returned to values not significantly different from the intact control level. Bilateral adrenalectomy caused an increase in both basal and acetylcholine-induced release of CRF and it is suggested that corticosteroids exert a negative feedback effect on the hypothalamus.
采用大鼠下丘脑体外制备方法，研究双侧肾上腺切除（有无替代疗法）对促肾上腺皮质激素释放因子（CRF）释放的影响。使用下丘脑损伤48小时的实验大鼠评估促肾上腺皮质激素释放因子，并将切除肾上腺的皮质酮生成作为终点指标。双侧肾上腺切除导致术后2小时内下丘脑CRF含量减少，但皮质酮替代疗法可预防此效应。此后，下丘脑CRF含量恢复至与完整对照水平无显著差异的值。双侧肾上腺切除导致CRF基础释放量和乙酰胆碱诱导释放量均增加，提示皮质类固醇对下丘脑发挥负反馈作用。
