Kurokawa T, Nonami T, Harada A, Nakao A, Sugiyama S, Ozawa T, Takagi H
Dept. of Surgery II, University of Nagoya School of Medicine, Japan.
Scand J Gastroenterol. 1991 Mar;26(3):269-74. doi: 10.3109/00365529109025041.
Interruption of hepatic blood supply for 60 min deteriorated liver mitochondrial respiratory functional indices--that is, respiratory control index (RCI) and the rate of oxygen consumption in state-III respiration (ST III O2). Recovery of ischemia-induced decreases in these functional indices in a saline-administered cirrhotic liver group was retarded compared with that in a normal liver group, and significantly low RCI and ST III O2 persisted 15 min after reperfusion. Prostaglandin E1 (PGE1) did not improve ischemia-induced decreases in RCI and ST III O2 but accelerated the recovery of mitochondrial respiratory function after reperfusion. Adenosine triphosphate (ATP) levels were markedly decreased during ischemia, and retardation of ATP recovery was also observed in rats with cirrhosis. PGE1 improved the recovery of ATP level in rats with cirrhosis. Liver blood flow in the cirrhotic liver was significantly lower than that of the normal liver. PGE1 enhanced liver blood flow. These results indicate that retardation of the recovery of RCI and ST III O2 in the cirrhotic liver might be based on the decrease in tissue blood flow and that agents increasing tissue blood flow might contribute to the acceleration of the recovery of mitochondrial respiratory function.
肝血流阻断60分钟会使肝线粒体呼吸功能指标恶化，即呼吸控制指数（RCI）和状态III呼吸时的氧消耗率（ST III O2）。与正常肝脏组相比，生理盐水处理的肝硬化肝脏组中缺血诱导的这些功能指标下降的恢复延迟，并且在再灌注后15分钟，RCI和ST III O2仍显著降低。前列腺素E1（PGE1）并未改善缺血诱导的RCI和ST III O2下降，但加速了再灌注后线粒体呼吸功能的恢复。缺血期间三磷酸腺苷（ATP）水平显著降低，肝硬化大鼠中也观察到ATP恢复延迟。PGE1改善了肝硬化大鼠的ATP水平恢复。肝硬化肝脏的肝血流量明显低于正常肝脏。PGE1增加了肝血流量。这些结果表明，肝硬化肝脏中RCI和ST III O2恢复延迟可能基于组织血流量减少，增加组织血流量的药物可能有助于加速线粒体呼吸功能的恢复。
