一种用于评估克罗恩病中脂肪和维生素B12吸收不良的三重同位素技术。
A triple isotope technique for estimation of fat and vitamin B12 malabsorption in Crohn's disease.
作者信息
Pedersen N T, Rannem T
机构信息
Medical Dept. A, Rigshospitalet, University of Copenhagen, Denmark.
出版信息
Scand J Gastroenterol. 1991 Mar;26(3):307-12. doi: 10.3109/00365529109025047.
A test for simultaneous estimation of vitamin B12 and fat absorption from stool samples was investigated in 25 patients with severe diarrhoea after operation for Crohn's disease. 51CrCl3 was ingested as a non-absorbable marker, 58Co-cyano-cobalamin as vitamin B12 tracer, and 14C-triolein as lipid tracer. Faeces were collected separately for 3 days. Some stool-to-stool variation in the 58Co/51Cr and 14C/51Cr ratios was seen. When the 58Co-B12 and 14C-triolein excretion was estimated in samples of the two stools with the highest activities of 51Cr, the variations of the estimates were less than +/- 10% and +/- 15% of the doses ingested, respectively. Twelve of the 25 patients were not able to collect faeces and urine quantitatively and separately. However, in all patients faeces with sufficient radioactivity for simultaneous estimation of faecal 58Co-B12 and 14C-triolein excretion from stool samples were obtained.
对25例克罗恩病术后出现严重腹泻的患者进行了一项用于同时测定粪便样本中维生素B12和脂肪吸收情况的试验。口服51CrCl3作为不可吸收标记物，58Co-氰钴胺作为维生素B12示踪剂，14C-三油酸甘油酯作为脂质示踪剂。分别收集3天的粪便。观察到58Co/51Cr和14C/51Cr比值在粪便之间存在一定差异。当在51Cr活性最高的两份粪便样本中估算58Co-维生素B12和14C-三油酸甘油酯的排泄量时，估算值的差异分别小于摄入剂量的±10%和±15%。25例患者中有12例无法分别定量收集粪便和尿液。然而，在所有患者中均获得了放射性足够的粪便样本，可用于同时估算粪便样本中58Co-维生素B12和14C-三油酸甘油酯的排泄量。
Zotero 插件