利用小麦胚芽的无细胞提取物合成仙台病毒多肽。
Synthesis of Sendai virus polypeptides by a cell-free extract from wheat germ.
作者信息
Davies J W, Portner A, Kingsbury D W
出版信息
J Gen Virol. 1976 Oct;33(1):117-23. doi: 10.1099/0022-1317-33-1-117.
PMID:185322
Abstract
The '18S' RNA population from Sendai virus-infected cells efficiently directed the synthesis of three virus-specific polypeptides P, NP and M, in wheat germ cell-free extracts. In agreement with previous results obtained with a reticulocyte extract, there was little or no production of virus glycopolypeptides. Analyses of tryptic peptides revealed close correspondence between the primary structures of NP and M made in vitro and the authentic virus polypeptides.
摘要
来自仙台病毒感染细胞的“18S”RNA群体在小麦胚芽无细胞提取物中有效地指导了三种病毒特异性多肽P、NP和M的合成。与先前用网织红细胞提取物获得的结果一致，几乎没有产生病毒糖多肽。胰蛋白酶肽分析表明，体外合成的NP和M的一级结构与真实病毒多肽之间有密切对应关系。
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