新城疫病毒多肽在小麦胚无细胞系统中的合成。
Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.
作者信息
Clinkscales C W, Bratt M A, Morrison T G
出版信息
J Virol. 1977 Apr;22(1):97-101. doi: 10.1128/JVI.22.1.97-101.1977.
Abstract
We have isolated 18S RNA from cytoplasmic extracts of Newcastle disease virus-infected Chinese hamster ovary cells and tested its ability to direct protein synthesis in extracts derived from wheat germ. The products of the cell-free reaction directed by this RNA contain polypeptides that comigrate with NP, M,F, and 47K roteins from virions. In addition, the products contain a polypeptide (67K) that migrates on polyacrylamide gels slightly faster than the HN protein from virions. Tryptic peptide analysis of the cell-free products and proteins from virions confirms their identity.
摘要
我们从感染新城疫病毒的中国仓鼠卵巢细胞的细胞质提取物中分离出18S RNA，并测试了其在小麦胚芽提取物中指导蛋白质合成的能力。由这种RNA指导的无细胞反应产物包含与病毒粒子中的NP、M、F和47K蛋白迁移率相同的多肽。此外，产物还包含一种多肽（67K），其在聚丙烯酰胺凝胶上的迁移速度比病毒粒子中的HN蛋白略快。对无细胞产物和病毒粒子蛋白进行的胰蛋白酶肽分析证实了它们的同一性。
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本文引用的文献
1
Bacteriophage f2 RNA: control of translation and gene order.噬菌体f2 RNA：翻译控制与基因顺序
Nature. 1968 Oct 26;220(5165):345-50. doi: 10.1038/220345a0.
2
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
3
Major human erythrocyte glycoprotein spans the cell membrane.主要的人类红细胞糖蛋白跨越细胞膜。
Nat New Biol. 1971 Jun 23;231(25):229-32. doi: 10.1038/newbio231229a0.
4
Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus.副粘病毒的蛋白质和糖蛋白：猴病毒5型、新城疫病毒和仙台病毒的比较
J Virol. 1971 Jan;7(1):47-52. doi: 10.1128/JVI.7.1.47-52.1971.
5
Inhibition of replication of ribonucleic acid bacteriophage f2 by superinfection with bacteriophage T4.噬菌体T4的超感染对核糖核酸噬菌体f2复制的抑制作用。
J Virol. 1971 Oct;8(4):417-29. doi: 10.1128/JVI.8.4.417-429.1971.
6
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.核糖核蛋白复合物的镁沉淀。分离未降解多核糖体和信使核糖核酸的便捷技术。
Biochemistry. 1974 Aug 13;13(17):3606-15. doi: 10.1021/bi00714a032.
7
Polyadenylate sequences on Newcastle disease virus mRNA synthesized in vivo and in vitro.新城疫病毒在体内和体外合成的信使核糖核酸上的聚腺苷酸序列。
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8
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9
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10
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J Virol. 1973 Sep;12(3):579-87. doi: 10.1128/JVI.12.3.579-587.1973.
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