Bellavia Daniele, Cellura Doriana, Sisino Giorgia, Barbieri Rainer
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, 90128 Palermo, Italy.
Anal Biochem. 2008 Aug 15;379(2):211-2. doi: 10.1016/j.ab.2008.05.010. Epub 2008 May 17.
We have developed a simple and inexpensive device to obtain linear sucrose gradients with commonly used laboratory materials--a syringe, a flask, a plastic tube, and a piece of Pongo (Play-Doh). Refractive index values measured on sucrose fractions collected using our system demonstrate both the linearity and reliability of the gradients obtained.
