Merkel H, Muth V
Biol Chem Hoppe Seyler. 1985 Dec;366(12):1097-102. doi: 10.1515/bchm3.1985.366.2.1097.
The separation of macromolecules in vertical rotors using linear or isokinetic sucrose gradients permits shorter centrifugation times and a higher sample capacity compared to swing-out rotors. In vertical rotors appropriate gradients give a resolution almost identical to that in swinging bucket rotors. This could be demonstrated for the isolation of polysomes and oligonucleosomes from rat liver. A microprocessor-controlled gradient former is presented which produces gradients of any desired shape. This device has been applied to prepare gradients with the desired linear or isokinetic shape after reorientation in the vertical tube during centrifugation.
与甩平式转头相比，使用线性或等速蔗糖梯度在垂直转头中分离大分子可缩短离心时间并提高样品容量。在垂直转头中，合适的梯度所产生的分辨率几乎与甩液桶转头相同。这一点已在从大鼠肝脏中分离多核糖体和寡核小体的过程中得到证实。本文介绍了一种微处理器控制的梯度形成器，它能产生任何所需形状的梯度。该装置已用于在离心过程中重新定位到垂直管后制备具有所需线性或等速形状的梯度。
