Enquobahrie Daniel A, Meller Margaret, Rice Kenneth, Psaty Bruce M, Siscovick David S, Williams Michelle A
Cardiovascular Health Research Unit, University of Washington, Seattle, WA, USA.
Am J Obstet Gynecol. 2008 Nov;199(5):566.e1-11. doi: 10.1016/j.ajog.2008.04.020. Epub 2008 Jun 4.
Candidate genes that are associated with preeclampsia have not been described fully. We conducted microarray and confirmatory quantitative real time polymerase chain reaction studies to investigate global placental gene expression in preeclampsia.
RNA was extracted from placental samples that were collected from 18 preeclampsia cases and 18 normotensive control subjects. Oligonucleotide probes that represented 22,000 genes were used to measure gene expression in each sample. Differential gene expression was evaluated with the Student t test, fold change assessment, and significance analysis of microarrays. Functions and functional relationships of differentially expressed genes were evaluated.
Genes (n = 58) that participated in immune system, inflammation, oxidative stress, signaling, growth, and development pathways were expressed differentially in preeclampsia. These genes included previously described candidate genes (such as leptin), potential candidate genes with related functions (such as CYP11A) and novel genes (such as CDKN1C).
Expression of genes (both candidate and novel) with diverse functions is associated with preeclampsia risk, which reflects the complex pathogenesis.
与子痫前期相关的候选基因尚未得到充分描述。我们进行了微阵列和验证性定量实时聚合酶链反应研究，以调查子痫前期胎盘基因的整体表达情况。
从18例子痫前期病例和18例血压正常的对照受试者采集的胎盘样本中提取RNA。使用代表22000个基因的寡核苷酸探针来测量每个样本中的基因表达。采用学生t检验、倍数变化评估和微阵列显著性分析来评估基因表达差异。对差异表达基因的功能及功能关系进行了评估。
参与免疫系统、炎症、氧化应激、信号传导、生长和发育途径的基因（n = 58）在子痫前期表达存在差异。这些基因包括先前描述的候选基因（如瘦素）、具有相关功能的潜在候选基因（如CYP11A）和新基因（如CDKN1C）。
具有多种功能的基因（包括候选基因和新基因）的表达与子痫前期风险相关，这反映了其复杂的发病机制。
