Aleixo Denise Lessa, Ferraz Fabiana Nabarro, Ferreira Erika Cristina, de Lana Marta, Gomes Mônica Lúcia, de Abreu Filho Benício Alves, de Araújo Silvana Marques
Laboratory of Parasitology, Universidade Estadual de Maringá, Maringá, PR, Brazil.
BMC Res Notes. 2012 Jul 11;5:352. doi: 10.1186/1756-0500-5-352.
There is no published information about the use of different protocols to administer a highly diluted medication.Evaluate the effect of different protocols for treatment with biotherapic T. cruzi 17 dH (BIOT(Tc17dH)) on clinical/parasitological evolution of mice infected with T. cruzi-Y strain.
A blind, randomized controlled trial was performed twice, using 60 28-day-old male Swiss mice infected with T. cruzi-Y strain, in five treatment groups: CI - treated with a 7% ethanol-water solution, diluted in water (10 μL/mL) ad libitum; BIOT(PI) - treated with BIOT(Tc17dH) in water (10 μL/mL) ad libitum during a period that started on the day of infection; BIOT(4DI) - treated with BIOT(Tc17dH) in water (10 μL/mL) ad libitum beginning on the 4th day of infection; BIOT(4-5-6) - treated with BIOT(Tc17dH) by gavage (0.2 mL/ animal/day) on the 4th, 5th and 6th days after infection; BIOT(7-8-9) - treated with BIOT(Tc17dH) by gavage (0.2 mL/ animal/day) on the 7th, 8th and 9th days after infection. We evaluated: parasitemia; total parasitemia (P(total)); maximum peak of parasites; prepatent period (PPP) - time from infection to detection of the parasite in blood; patent period (PP) - period when the parasitemia can be detected in blood; clinical aspects; and mortality.
Parasitological parameters in the BIOT(PI) and mainly in the BIOT(4PI) group showed better evolution of the infection compared to the control group (CI), with lower P(total), lower maximum peak of parasites, higher PPP, lower PP and longer survival times. These animals showed stable body temperature and higher weight gain and water consumption, with more animals having normal-appearing fur for longer periods. In contrast, groups BIOT(4-5-6) and BIOT(7-8-9) showed worse evolution of the infection compared to the control group, considering both parasitological and clinical parameters. The correlation analysis combined with the other data from this study indicated that the prepatent period is the best parameter to evaluate the effect of a medication in this model.
The BIOT(4DI) group showed the best clinical and parasitological evolution, with lower parasitemia and a trend toward lower mortality and a longer survival period. The prepatent period was the best parameter to evaluate the effect of a medication in this model.
尚无关于使用不同方案给予高度稀释药物的公开信息。评估不同方案用生物疗法克氏锥虫17 dH（BIOT(Tc17dH)）治疗感染克氏锥虫-Y株小鼠的临床/寄生虫学演变情况。
进行了两次盲法随机对照试验，使用60只感染克氏锥虫-Y株的28日龄雄性瑞士小鼠，分为五个治疗组：CI组 - 用7%乙醇水溶液处理，在水中稀释（10 μL/mL）随意饮用；BIOT(PI)组 - 从感染当天开始，在水中（10 μL/mL）随意饮用BIOT(Tc17dH)进行治疗；BIOT(4DI)组 - 从感染第4天开始，在水中（10 μL/mL）随意饮用BIOT(Tc17dH)进行治疗；BIOT(4 - 5 - 6)组 - 在感染后第4、5和6天通过灌胃（0.2 mL/动物/天）给予BIOT(Tc17dH)进行治疗；BIOT(7 - 8 - 9)组 - 在感染后第7、8和9天通过灌胃（0.2 mL/动物/天）给予BIOT(Tc17dH)进行治疗。我们评估了：寄生虫血症；总寄生虫血症（P(总)）；寄生虫的最大峰值；潜伏期（PPP） - 从感染到血液中检测到寄生虫的时间；发病期（PP） - 血液中可检测到寄生虫血症的时期；临床情况；以及死亡率。
与对照组（CI）相比，BIOT(PI)组尤其是BIOT(4PI)组的寄生虫学参数显示感染情况有更好的演变，P(总)更低，寄生虫的最大峰值更低，PPP更长，PP更短，存活时间更长。这些动物体温稳定，体重增加和饮水量更高，更多动物在更长时间内毛发外观正常。相比之下，考虑寄生虫学和临床参数，BIOT(4 - 5 - 6)组和BIOT(7 - 8 - 9)组的感染演变情况比对照组更差。结合本研究的其他数据进行的相关分析表明，潜伏期是评估该模型中药物效果的最佳参数。
BIOT(4DI)组显示出最佳的临床和寄生虫学演变，寄生虫血症更低，死亡率有降低趋势且存活期更长。潜伏期是评估该模型中药物效果的最佳参数。
