Hao Huiling, Wei Jianrong, Dai Jianqing, Du Jiawei
Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Rd., Shanghai 200032, China.
J Med Entomol. 2008 May;45(3):533-9. doi: 10.1603/0022-2585(2008)45[533:habboa]2.0.co;2.
The changes of the host-seeking and blood-feeding behavior of Aedes albopictus (Skuse) (Diptera: Culicidae) surviving in a space containing vapors of the spatial repellents geraniol, eugenol, citral, anisaldehyde, or citronellal were evaluated using an arm-in-cage test and a bioassay of bloodmeals on a shaved mouse. The mosquitoes surviving concentrations of geraniol, citral, eugenol, or anisaldehyde at 0.013, 0.025, 0.050, 0.100, and 0.250 microg/cm3 for 24 and 48 h all showed different degrees of reduction in host-seeking ability. After 48 h of exposure to 0.250 microg/cm3 geraniol, almost 100% of the mosquitoes lost their host-seeking ability. The next most potent spatial repellent, anisaldehyde, stopped host seeking by > 85.5%. Citronellal did not result in a significant reduction in the host-seeking ability at any concentration level after either 24 or 48 h of treatment. We also found that reduction of host-seeking ability recovered after various times. The longest recovery time (144 h) was observed for geraniol after 24 h at 0.250 microg/cm3. In the study, geraniol, eugenol, and citral all significantly affected the activation and orientation stages of the blood-feeding behavior. However, only anisaldehyde significantly interrupted the normal blood-feeding of mosquitoes in all stages of behavior. These initial laboratory results clearly showed that anisaldehyde and geraniol could be promising spatial repellents against Ae. albopictus that they could play a major role in new repellent technology.
利用笼中手臂试验和在剃毛小鼠上进行血餐生物测定，评估了在含有香叶醇、丁香酚、柠檬醛、茴香醛或香茅醛等空间驱避剂蒸汽的空间中存活的白纹伊蚊（Skuse）（双翅目：蚊科）寻找宿主和吸血行为的变化。在0.013、0.025、0.050、0.100和0.250微克/立方厘米的香叶醇、柠檬醛、丁香酚或茴香醛存活浓度下暴露24小时和48小时的蚊子，其寻找宿主能力均呈现出不同程度的下降。暴露于0.250微克/立方厘米香叶醇48小时后，几乎100%的蚊子丧失了寻找宿主的能力。其次效力最强的空间驱避剂茴香醛，使寻找宿主行为停止的比例超过85.5%。在处理24小时或48小时后，香茅醛在任何浓度水平下均未导致寻找宿主能力的显著下降。我们还发现，寻找宿主能力的下降在不同时间后会恢复。在0.250微克/立方厘米暴露24小时后，香叶醇的最长恢复时间为144小时。在该研究中，香叶醇、丁香酚和柠檬醛均显著影响吸血行为的激活和定向阶段。然而，只有茴香醛在行为的所有阶段均显著干扰了蚊子的正常吸血。这些初步的实验室结果清楚地表明，茴香醛和香叶醇有望成为针对白纹伊蚊的空间驱避剂，它们可在新型驱避技术中发挥主要作用。
