Dandekar Deepak V, Jayaprakasha Guddadarangavvanahally K, Patil Bhimanagouda S
Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77845, USA.
Z Naturforsch C J Biosci. 2008 Mar-Apr;63(3-4):176-80. doi: 10.1515/znc-2008-3-403.
Citrus limonoids were demonstrated to possess potential biological activities in reducing the risk of certain diseases. Limonoids are present in citrus fruits in the form of aglycones and glucosides. At present, limonoid aglycones and limonoid glucosides are extracted in multiple steps using different solvents. In order to understand their potential bioactivity, it may be beneficial to isolate and purify these compounds using environment friendly methods. A new method of extraction and purification of limonoids was established using a hydrotrope polystyrene adsorbent resin. Extraction of aglycones and glucosides was achieved in a single step, using an aqueous solution of sodium cumene sulphonate (Na-CuS). Sour orange (Citrus aurantium L.) seed powder was extracted with 2 M Na-CuS solution at 45 degrees C for 6 h. The filtered extract was diluted with water and loaded on an SP 700 adsorbent column. The column was washed with distilled water to remove the hydrotrope and then eluted using water and methanol in different compositions to obtain three compounds. The structures of the isolated compounds were confirmed by NMR spectroscopy as deacetyl nomilinic acid glucoside (DNAG), deacetyl nomilin (DAN) and limonin (LIM).
柑橘类柠檬苦素被证明在降低某些疾病风险方面具有潜在的生物活性。柠檬苦素以苷元和糖苷的形式存在于柑橘类水果中。目前，柠檬苦素苷元和柠檬苦素糖苷是使用不同溶剂通过多步提取的。为了了解它们的潜在生物活性，使用环境友好的方法分离和纯化这些化合物可能是有益的。利用水溶助长剂聚苯乙烯吸附树脂建立了一种新的柠檬苦素提取和纯化方法。使用异丙苯磺酸钠（Na-CuS）水溶液一步实现苷元和糖苷的提取。用2 M Na-CuS溶液在45℃下提取酸橙（Citrus aurantium L.）种子粉6小时。将过滤后的提取物用水稀释并加载到SP 700吸附柱上。用蒸馏水洗涤柱子以除去水溶助长剂，然后使用不同组成的水和甲醇洗脱以获得三种化合物。通过核磁共振光谱确认分离出的化合物的结构为脱乙酰诺米林酸葡萄糖苷（DNAG）、脱乙酰诺米林（DAN）和柠檬苦素（LIM）。
