Unno N, Nishiyama M, Suzuki M, Yamamoto N, Inuzuka K, Sagara D, Tanaka H, Konno H
Division of Vascular Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Second Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
Division of Vascular Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Second Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
Eur J Vasc Endovasc Surg. 2008 Aug;36(2):230-236. doi: 10.1016/j.ejvs.2008.04.013. Epub 2008 Jun 4.
A new diagnostic imaging technique that can assess lymph function is needed as a screening test in daily practice. This study assessed the use of indocyanine green (ICG) fluorescence lymphography in subjects without leg oedema.
0.3ml of ICG (0.5 %) was injected subcutaneously at the dorsum of the foot. Subsequently, the movement of ICG dye from the injection site to the groin was traced by visualizing its fluorescence signal with an infrared light camera. The time for the dye to reach the knee and groin were measured (Transit time to knee: TT(K), Transit time to groin: TT(G)). TT(G) was measured while standing, lying at a supine position, standing with massage, and sitting while using a cycle ergometer exercise at an intensity of 50W at 50rpm in ten healthy volunteers at intervals of 14 days.
Mean TT(G) during standing was 357+/-289 and 653+/-564 seconds for the right and left legs respectively. Compared to TT(G) in the standing position, all other conditions shortened TT(G). In another seventeen subjects without leg oedema, we compared transit time obtained with ICG fluorescence lymphography to that with dynamic lymphoscintigraphy. A significant correlation between transit time measured with ICG lymphography and dynamic lymphoscintigraphy was identified (r(2)=0.64, p<0.01).
ICG fluorescence lymphography has the potential to become an alternative lymphatic imaging technique to assess lymph function.
在日常实践中，需要一种能够评估淋巴功能的新型诊断成像技术作为筛查测试。本研究评估了吲哚菁绿（ICG）荧光淋巴造影术在无腿部水肿受试者中的应用。
将0.3ml的ICG（0.5%）皮下注射到足部背侧。随后，通过用红外光相机可视化其荧光信号，追踪ICG染料从注射部位到腹股沟的移动。测量染料到达膝盖和腹股沟的时间（到达膝盖的转运时间：TT(K)，到达腹股沟的转运时间：TT(G)）。在10名健康志愿者中，每隔14天测量一次TT(G)，测量时分别处于站立、仰卧、站立并按摩以及以50W、50rpm的强度使用自行车测力计进行坐姿运动的状态下。
站立时，右腿和左腿的平均TT(G)分别为357±289秒和653±564秒。与站立位的TT(G)相比，所有其他状态下TT(G)均缩短。在另外17名无腿部水肿的受试者中，我们将ICG荧光淋巴造影术获得的转运时间与动态淋巴闪烁造影术获得的转运时间进行了比较。结果发现ICG淋巴造影术测量的转运时间与动态淋巴闪烁造影术测量的转运时间之间存在显著相关性（r(2)=0.64，p<0.01）。
ICG荧光淋巴造影术有潜力成为一种评估淋巴功能的替代性淋巴成像技术。
