Alford R L, Honda S, Lawrence C B, Belmont J W
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
Virology. 1991 Aug;183(2):611-9. doi: 10.1016/0042-6822(91)90990-s.
We have examined the cis-acting RNA packaging signal (psi) from Moloney murine leukemia virus using a combination of chemical and primary sequence analysis techniques. For our chemical analyses, we used dimethyl sulfate, kethoxal, and 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluene sulfonate as probes for RNA secondary structure. The structural information obtained from these studies was used to constrain computer algorithms for prediction of RNA secondary structure. In addition, we generated and analyzed a phylogenetic comparison of homologous sequences from related retroviruses. From these data, we have developed two models for the RNA secondary structure of the packaging signal psi. Both of these models suggest the presence of secondary structure elements in a region of the psi RNA known to be required for function.
我们使用化学和一级序列分析技术相结合的方法，研究了莫洛尼鼠白血病病毒的顺式作用RNA包装信号（ψ）。在化学分析中，我们使用硫酸二甲酯、乙二醛和1-环己基-3-（2-吗啉代乙基）碳二亚胺对甲苯磺酸盐作为RNA二级结构的探针。从这些研究中获得的结构信息被用于限制预测RNA二级结构的计算机算法。此外，我们生成并分析了相关逆转录病毒同源序列的系统发育比较。根据这些数据，我们开发了两种包装信号ψ的RNA二级结构模型。这两种模型都表明，在已知对功能有要求的ψ RNA区域存在二级结构元件。
