Firth Andrew E, Atkins John F
BioSciences Institute, University College Cork, Cork, Ireland.
Arch Virol. 2008;153(7):1379-83. doi: 10.1007/s00705-008-0119-5. Epub 2008 Jun 6.
The genus Waikavirus belongs to the order Picornavirales, whose members all use a polyprotein expression strategy. With the exception of Theiler's virus, overlapping genes are essentially unknown in the order. Recently, we reported experimental verification for a new short overlapping coding sequence (CDS) in the Potyviridae-a family in which overlapping genes were previously unknown. Using the same bioinformatics software (MLOGD), we have identified an approximately 89-codon conserved open reading frame (ORF) with a strong coding signature in members of the genus Waikavirus. The ORF overlaps the polyprotein ORF but is in the +1 reading frame. Here, we describe the bioinformatic analysis.
怀卡病毒属属于小RNA病毒目，该目的成员均采用多聚蛋白表达策略。除了泰勒氏病毒外，该目中基本不存在重叠基因。最近，我们报道了对马铃薯Y病毒科中一个新的短重叠编码序列（CDS）的实验验证，该科此前未知存在重叠基因。使用相同的生物信息学软件（MLOGD），我们在怀卡病毒属成员中鉴定出一个约89个密码子的保守开放阅读框（ORF），具有很强的编码特征。该ORF与多聚蛋白ORF重叠，但处于+1阅读框。在此，我们描述生物信息学分析。
