鉴定一株中国三叶海桐中新的 Waikavirus 病毒。
Identification of a novel waikavirus infecting Pittosporum tobira in China.
机构信息
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China.
Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, Liaoning, 125100, China.
出版信息
Arch Virol. 2024 Jun 12;169(7):144. doi: 10.1007/s00705-024-06068-6.
A novel waikavirus, tentatively named "Pittosporum tobira waikavirus" (PtWV), was identified in Pittosporum tobira plants exhibiting mosaic and ringspot symptoms on foliage in Yunnan, China. The full-length genomic sequence was determined by high-throughput sequencing and rapid amplification of cDNA ends. The genome of PtWV is 12,709 nt in length and has a large open reading frame (ORF) of 11,010 nt, encoding a polyprotein, and a small ORF that encodes a 13.2-kDa bellflower vein chlorosis virus (BVCV)-like protein. Phylogenetic analysis and sequence alignment revealed that PtWV is closely related to actinidia yellowing virus 1 (AcYV1), which shares the highest amino acid (aa) sequence similarity (50.1% identity) in the Pro-RdRp region. To the best of our knowledge, this is the first report of a novel waikavirus in P. tobira.
在中国云南，表现为花叶斑驳和环斑症状的叶杜鹃属植物中发现了一株新的 waikavirus，暂命名为“叶杜鹃 waikavirus”（PtWV）。通过高通量测序和快速扩增 cDNA 末端，确定了全基因组序列。PtWV 的基因组长度为 12709nt，具有一个长的开放阅读框（ORF），长 11010nt，编码一个多聚蛋白，以及一个小的 ORF，编码一个 13.2kDa 的矮牵牛叶脉褪绿病毒（BVCV）样蛋白。系统进化分析和序列比对表明，PtWV 与猕猴桃黄化病毒 1（AcYV1）密切相关，在 Pro-RdRp 区具有最高的氨基酸（aa）序列相似性（50.1% identity）。据我们所知，这是 P. tobira 中首例报道的新型 waikavirus。
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