Nakamura Akari, Sakano Takashi, Nakayama Tetsuo, Shimoda Hiroko, Okada Yasuyuki, Hanayama Ryuzo, Nomoto Katsuhiro, Suto Tetsushi, Kinoshita Yoshihisa, Furue Takeki, Ono Hiroaki, Ohta Toshiyuki
Department of Pediatrics, National Hospital Organization, Higashi-Hiroshima Medical Center, Higashi-Hiroshima, Japan.
Eur J Pediatr. 2009 Mar;168(3):347-9. doi: 10.1007/s00431-008-0744-8. Epub 2008 Jun 6.
We report a 28-day-old female infant with pertussis presenting as severe acute bronchiolitis with cyanosis. On admission, the patient's symptoms were similar to those of acute bronchiolitis. However, occasional apneic episodes with cyanosis and peripheral lymphocytosis suggested neonatal pertussis and prompted us to examine the presence of Bordetella pertussis using loop-mediated isothermal amplification (LAMP) based on the insertion sequence IS481. LAMP of the nasopharyngeal and intratracheal aspirates was positive for B. pertussis and a diagnosis of neonatal pertussis was made. As the clinical features of pertussis in neonates and early infancy are not characteristic, LAMP is a useful tool for rapid diagnosis of B. pertussis infection.
我们报告了一名28日龄的患百日咳的女婴，表现为伴有发绀的严重急性细支气管炎。入院时，患者的症状与急性细支气管炎相似。然而，偶尔出现的伴有发绀的呼吸暂停发作和外周淋巴细胞增多提示新生儿百日咳，并促使我们基于插入序列IS481，使用环介导等温扩增技术（LAMP）检测百日咳博德特氏菌的存在。鼻咽和气管内吸出物的LAMP检测显示百日咳博德特氏菌呈阳性，从而做出了新生儿百日咳的诊断。由于新生儿和婴儿早期百日咳的临床特征不典型，LAMP是快速诊断百日咳博德特氏菌感染的有用工具。
