Blackler Adele R, Speers Anna E, Ladinsky Mark S, Wu Christine C
Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA.
J Proteome Res. 2008 Jul;7(7):3028-34. doi: 10.1021/pr700795f. Epub 2008 Jun 7.
Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome.
整合膜蛋白执行关键的细胞功能,并且是大多数药物的作用靶点。然而,其膜嵌入结构域的疏水性使得它们难以进行研究。在此,我们描述了一种鸟枪法蛋白质组学方法,用于对整合膜蛋白的膜嵌入跨膜结构域进行高通量分析,该方法扩展了膜蛋白质组的覆盖深度。