The Pasarow Mass Spectrometry Laboratory, The NPI-Semel Institute for Neuroscience and Human Behavior, The David Geffen School of Medicine, University of California, Los Angeles, 760 Westwood Plaza, Los Angeles, California 90095, United States.
Anal Chem. 2013 Mar 5;85(5):2558-68. doi: 10.1021/ac303064a. Epub 2013 Feb 19.
Integral membrane proteins reside within the bilayer membranes that surround cells and organelles, playing critical roles in movement of molecules across them and the transduction of energy and signals. While their extreme amphipathicity presents technical challenges, biological mass spectrometry has been applied to all aspects of membrane protein chemistry and biology, including analysis of primary, secondary, tertiary, and quaternary structures as well as the dynamics that accompany functional cycles and catalysis.
整合膜蛋白位于包围细胞和细胞器的双层膜内，在分子跨膜运动和能量及信号转导中发挥关键作用。尽管它们的极端两亲性带来了技术挑战，但生物质谱已被应用于膜蛋白化学和生物学的各个方面，包括分析一级、二级、三级和四级结构以及伴随功能循环和催化的动力学。
