Simon-Colin Christelle, Raguénès Gérard, Crassous Philippe, Moppert Xavier, Guezennec Jean
Laboratoire de Biotechnologie et Molécules Marines, Institut Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, BIOMAR/BMM, Plouzané, France.
Int J Biol Macromol. 2008 Aug 15;43(2):176-81. doi: 10.1016/j.ijbiomac.2008.04.011. Epub 2008 May 2.
Pseudomonas guezennei biovar. tikehau was isolated from a microbial mat on the atoll of Tikehau in French Polynesia, and is able to synthesize medium chain length poly-beta-hydroxyalkanaote copolymers when grown on coprah oil. A two-step cultivation process was used and the biosynthesis of PHAs was followed along 52h by regular culture sampling. The polyester was purified from freeze-dried cells and analysed by nuclear magnetic resonance (NMR), Fourier transform infra red (FTIR), and gas chromatography mass spectrometries. The copolyester produced by P. guezennei biovar. tikehau from coprah oil mainly consisted of saturated monomers, i.e. 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), and the monomeric composition of the polyester did not change during the fermentation process. However, yield of PHAs production varied from 4% of the cellular dry weight (CDW) to 63% obtained after 36h. Scan electron microscopy was used to study the morphology and organization of PHAs granules within the cells and revealed the presence of several granules occupying almost the entire cell volume.
蒂凯豪岛假单胞菌生物变种tikehau是从法属波利尼西亚蒂凯豪环礁的微生物垫中分离出来的，当在椰干油上生长时，它能够合成中链长度的聚-β-羟基链烷酸酯共聚物。采用两步培养法，并通过定期培养取样，对PHA的生物合成进行了52小时的跟踪。从冻干细胞中纯化聚酯，并通过核磁共振（NMR）、傅里叶变换红外光谱（FTIR）和气相色谱质谱进行分析。蒂凯豪岛假单胞菌生物变种tikehau从椰干油中产生的共聚酯主要由饱和单体组成，即3-羟基辛酸（3HO）和3-羟基癸酸（3HD），并且在发酵过程中聚酯的单体组成没有变化。然而，PHA的产量从细胞干重（CDW）的4%到36小时后获得的63%不等。扫描电子显微镜用于研究细胞内PHA颗粒的形态和组织，并揭示了存在几个几乎占据整个细胞体积的颗粒。
