Simon-Colin C, Alain K, Raguénès G, Schmitt S, Kervarec N, Gouin C, Crassous P, Costa B, Guezennec J G
Institut Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, BIOMAR/BMM, B.P. 70, 29280 Plouzané, France.
Bioresour Technol. 2009 Dec;100(23):6033-9. doi: 10.1016/j.biortech.2009.06.075. Epub 2009 Jul 25.
A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-beta-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6-C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point T(m) near 50 degrees C, enthalpy of fusion DeltaH(m) from 27 to 32 J/g, and glass transition temperature T(g) of -43 degrees C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus and the name of Pseudomonas raguenesii sp. nov. is proposed.
从法属波利尼西亚的微生物垫中分离出一种新细菌，命名为TE9菌株，并对其在化妆品副产品培养过程中合成中链长度聚-β-羟基脂肪酸酯（mcl-PHAs）的能力进行了研究。通过气相色谱-质谱联用（GC/MS）、核磁共振（NMR）和傅里叶变换红外光谱（FTIR）对PHA的组成进行了分析。PHA由C6-C14 3-羟基酸单体组成，其中3-羟基辛酸（3HO）、3-羟基癸酸（3HD）和3-羟基十二烷酸（3HDD）占主导。差示扫描量热法（DSC）实验对熔点T(m)接近50℃、熔化焓ΔH(m)为27至32 J/g、玻璃化转变温度T(g)为-43℃的弹性体材料进行了表征。分子量范围为175,000至358,000 g/mol。基于表型特征和基因研究，TE9菌株被归入假单胞菌属，并提出了新种拉圭内斯假单胞菌（Pseudomonas raguenesii sp. nov.）的名称。
