Moura M J, Ribeiro B, Sousa J, Costa-Ferreira M
Bioengineering and Bioprocesses, Unit-INETI, Estrada do Paço do Lumiar, 22, P-1649-038 Lisboa, Portugal.
Bioresour Technol. 2008 Dec;99(18):8840-3. doi: 10.1016/j.biortech.2008.04.043. Epub 2008 Jun 5.
Sulfur-oxidising acidophilic bacteria were obtained from weathered sulfur piles produced by a petroleum refinery. When grown on commercial sulfur the yield was 10(10)cell/g S. Sulfur conversion to sulfate was about 95% after 17 days. Cultures were also grown together with ash obtained from incinerated refinery sludge, which contained high amounts of iron. Cultures grown in ash plus sulfur gave somewhat higher values for the growth parameters (Y=1.6 x10(10)cell/g S). The sulfur conversion was about 70% (after 17 days) and more than 90% of the iron present in the ash was also leached. The sulfur-reduced compound thiosulfate, determined using ion pair HPLC, was found in the presence and absence of ash although the profile was different in each case. Sulfite was only found in non-ash containing cultures, whereas tetrathionate was only formed in the presence of ash. These results are discussed with reference to the substrates used by the micro-organisms.
硫氧化嗜酸菌取自一家炼油厂产生的风化硫堆。在商业硫上生长时，产量为10(10)个细胞/克硫。17天后，硫转化为硫酸盐的转化率约为95%。培养物还与焚烧炼油厂污泥所得的灰分一起培养，该灰分含有大量铁。在灰分加硫中生长的培养物的生长参数值略高（Y = 1.6×10(10)个细胞/克硫）。硫转化率约为70%（17天后），灰分中存在的铁有90%以上也被浸出。使用离子对高效液相色谱法测定，无论有无灰分，均发现了硫还原化合物硫代硫酸盐，尽管每种情况下的图谱不同。亚硫酸盐仅在不含灰分的培养物中发现，而连四硫酸盐仅在有灰分的情况下形成。结合微生物使用的底物对这些结果进行了讨论。
