Cosma Pinalysa, Fini Paola, Rochira Sergio, Catucci Lucia, Castagnolo M, Agostiano Angela, Gristina Roberto, Nardulli Marina
Dip. di Chimica, Università di Bari, Via Orabona 4, 70126 Bari, Italy.
Bioelectrochemistry. 2008 Nov;74(1):58-61. doi: 10.1016/j.bioelechem.2008.04.020. Epub 2008 Apr 27.
The aggregation status of chlorophyll a (Chl a) and the ability of four cyclodextrins, hydroxypropyl-beta-cyclodextrin (HP-beta-CD), hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD), heptakis(2,6-di-O-methyl)-beta-cyclodextrin (DIMEB), and heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin (TRIMEB), to solubilize the pigment in the complete cellular medium RPMI 1640 was estimated by means of UV-Vis absorption and static resonance light scattering (RLS) measurements. The results indicate that the pigment interacts with cyclodextrins in the cellular medium differently to that observed in water. The cytotoxic and phototoxic activity of these complexes towards human leukemia T-lymphocytes (Jurkat cells) was tested by means of experiments aimed to discriminate between the intrinsic toxicity and the toxicity induced by light. The overall data indicate that the HP-beta-CD is the cyclodextrins having the best characteristics to form with Chl a a potential supramolecular system for the photodynamic therapy.
通过紫外可见吸收和静态共振光散射（RLS）测量，评估了叶绿素a（Chl a）的聚集状态以及四种环糊精，即羟丙基-β-环糊精（HP-β-CD）、羟丙基-γ-环糊精（HP-γ-CD）、七（2,6-二-O-甲基）-β-环糊精（DIMEB）和七（2,3,6-三-O-甲基）-β-环糊精（TRIMEB）在完全细胞培养基RPMI 1640中溶解该色素的能力。结果表明，该色素在细胞培养基中与环糊精的相互作用不同于在水中观察到的情况。通过旨在区分内在毒性和光诱导毒性的实验，测试了这些复合物对人白血病T淋巴细胞（Jurkat细胞）的细胞毒性和光毒性活性。总体数据表明，HP-β-CD是与Chl a形成用于光动力治疗的潜在超分子体系的特性最佳 的环糊精。
