Chu C R, Izzo N J, Coyle C H, Papas N E, Logar A
Cartilage Restoration Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, School of Medicine, 3471 Fifth Ave, Suite 911, Pittsburgh, Pennsylvania 15213, USA.
J Bone Joint Surg Br. 2008 Jun;90(6):814-20. doi: 10.1302/0301-620X.90B6.20079.
We have studied the effects of bupivacaine on human and bovine articular chondrocytes in vitro. Time-lapse confocal microscopy of human articular chondrocytes showed > 95% cellular death after exposure to 0.5% bupivacaine for 30 minutes. Human and bovine chondrocytes exposed to 0.25% bupivacaine had a time-dependent reduction in viability, with longer exposure times resulting in higher cytotoxicity. Cellular death continued even after removal of 0.25% bupivacaine. After exposure to 0.25% bupivacaine for 15 minutes, flow cytometry showed bovine chondrocyte viability to be 41% of saline control after seven days. After exposure to 0.125% bupivacaine for up to 60 minutes, the viability of both bovine and human chondrocytes was similar to that of control groups. These data show that prolonged exposure 0.5% and 0.25% bupivacaine solutions are potentially chondrotoxic.
我们已经在体外研究了布比卡因对人及牛关节软骨细胞的影响。对人关节软骨细胞进行延时共聚焦显微镜观察显示，在暴露于0.5%布比卡因30分钟后，超过95%的细胞死亡。暴露于0.25%布比卡因的人及牛软骨细胞活力呈时间依赖性降低，暴露时间越长，细胞毒性越高。即使去除0.25%布比卡因后，细胞死亡仍在继续。在暴露于0.25%布比卡因15分钟后，流式细胞术显示7天后牛软骨细胞活力为生理盐水对照组的41%。在暴露于0.125%布比卡因长达60分钟后，牛和人软骨细胞的活力均与对照组相似。这些数据表明，长时间暴露于0.5%和0.25%布比卡因溶液可能具有软骨毒性。
