Brosig Alexander, Nesper Jutta, Welte Wolfram, Diederichs Kay
Department of Biology, University of Konstanz, 78457 Konstanz, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jun 1;64(Pt 6):533-6. doi: 10.1107/S1744309108013602. Epub 2008 May 23.
The cell envelope of the thermophilic bacterium Thermus thermophilus is multilayered and includes an outer membrane with integral outer membrane proteins that are not well characterized. The hypothetical protein TTC0834 from T. thermophilus HB27 was identified as a 22 kDa outer membrane protein containing eight predicted beta-strands. TTC0834 was expressed with an N-terminal His tag in T. thermophilus HB8 and detected in the S-layer/outer membrane envelope fraction. His-TTC0834 was purified and crystallized under various conditions. Native data sets were collected to 3.2 A resolution and the best diffracting crystals belonged to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 166.67, c = 97.53 A.
嗜热栖热菌的细胞包膜是多层的,包括一层外膜,外膜上有一些整合的外膜蛋白,但对这些蛋白的特性了解并不充分。嗜热栖热菌HB27中的假定蛋白TTC0834被鉴定为一种22 kDa的外膜蛋白,含有8个预测的β链。TTC0834在嗜热栖热菌HB8中以N端带有His标签的形式表达,并在S层/外膜包膜组分中被检测到。His-TTC0834在各种条件下被纯化并结晶。收集了分辨率为3.2 Å的原生数据集,衍射效果最好的晶体属于空间群P3(1)21或P3(2)21,晶胞参数a = b = 166.67,c = 97.53 Å。