Ohzeki M, Yaoi T, Moriyama H, Oshima T, Tanaka N
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama.
J Biochem. 1995 Oct;118(4):679-80. doi: 10.1093/oxfordjournals.jbchem.a124963.
Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant. Pillar-like crystals of about 0.6 x 0.6 x 0.3 mm3 were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I2(1)2(1)2(1) space group with unit cell dimensions of a = 100.1 A, b = 150.4 A, and c = 87.4 A. Intensity data were collected up to 2.5 A resolution.
嗜热栖热菌HB8的异柠檬酸脱氢酶通过气相扩散悬滴法，以聚乙二醇6000作为沉淀剂进行结晶。得到了尺寸约为0.6×0.6×0.3 mm³的柱状晶体。对一系列振荡照片的分析表明，正交晶体属于I222或I2(1)2(1)2(1)空间群，晶胞参数为a = 100.1 Å，b = 150.4 Å，c = 87.4 Å。收集了分辨率高达2.5 Å的强度数据。
