Morozov I I, Petin V G
Tsitologiia. 2008;50(2):182-6.
The influence of the length of staying of Escherichia coli B/r cells in hypertonic NaCl solution before heating at 52 and 60 degrees C on the magnitude of salt thermoprotection was investigated. In addition, the dependence of the isotonic and thermoprotective NaCl concentrations on the exposure temperature was investigated. It was shown that the volume of cell osmotic thermoprotection was independent on the length of preliminary staying of microorganisms in hypertonic NaCl solution. It was also shown that the magnitude of isotonic and thermoprotective osmolite concentrations increased with the increase in the exposure temperature. The analysis of the data obtained and published in literature indicates that the compensating mechanism is involved in salt bacteria thermoprotection rather than the dehydratation one.
研究了大肠杆菌B/r细胞在52℃和60℃加热前于高渗NaCl溶液中的停留时间对盐热保护程度的影响。此外,还研究了等渗和热保护NaCl浓度对暴露温度的依赖性。结果表明,细胞渗透热保护的体积与微生物在高渗NaCl溶液中的预停留时间无关。还表明,等渗和热保护渗透剂浓度的大小随暴露温度的升高而增加。对所获得的数据以及文献中发表的数据进行分析表明,盐细菌的热保护涉及补偿机制而非脱水机制。