Mata J A, Béjar V, Bressollier P, Tallon R, Urdaci M C, Quesada E, Llamas I
Department of Microbiology, Microbial Exopolysaccharide Research Group, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja, Granada, Spain.
J Appl Microbiol. 2008 Aug;105(2):521-8. doi: 10.1111/j.1365-2672.2008.03789.x. Epub 2008 Jun 5.
To study the exopolysaccharides (EPSs) produced by three novel moderately halophilic species belonging to the family Alteromonadaceae to optimize EPS yields, characterize their physical and chemical properties and evaluate possible biotechnological applications for these polymers.
EPSs synthesized by Idiomarina fontislapidosi F32(T), Idiomarina ramblicola R22(T) and Alteromonas hispanica F23(T) were collected and analysed under optimum conditions: MY medium supplemented with 7.5% (w/v) salts; 32 degrees C; and 1% (w/v) glucose. Polymers were synthesized mainly during the early stationary growth phase with yields ranging from 1 to 1.5 g l(-1). The Idiomarina species each produced an anionic EPS composed mainly of glucose, mannose and galactose. A. hispanica synthesized an anionic EPS composed mainly of glucose, mannose and xylose. Solutions of all the polymers were low in viscosity and pseudoplastic in their behaviour. They showed emulsifying activity and the capacity to bind some metals.
The Alteromonadaceae species studied in this work produced EPSs with physical and chemical properties different from those produced by other halophilic and nonhalophilic bacteria, suggesting that the wide diversity of micro-organisms being encountered nowadays in hypersaline environments offers enormous potential resources for biotechnological applications.
We have optimized the EPS production and analysed new biopolymers produced by some recently described, moderately halophilic bacteria. These biopolymers are chemically and physically different from others already in use in biotechnology and offer hopes for new applications, especially in the case of A. hispanica, which may prove to be a viable source of xylo-oligosaccharides.
研究属于交替单胞菌科的三种新型中度嗜盐菌产生的胞外多糖（EPSs），以优化EPS产量，表征其物理和化学性质，并评估这些聚合物可能的生物技术应用。
收集并分析了由石礁海杆菌F32(T)、皱波海杆菌R22(T)和西班牙交替单胞菌F23(T)在最佳条件下合成的EPSs：添加7.5%（w/v）盐的MY培养基；32℃；1%（w/v）葡萄糖。聚合物主要在生长稳定期早期合成，产量范围为1至1.5 g l(-1)。每种海杆菌属菌株都产生一种主要由葡萄糖、甘露糖和半乳糖组成的阴离子型EPS。西班牙交替单胞菌合成了一种主要由葡萄糖、甘露糖和木糖组成的阴离子型EPS。所有聚合物溶液的粘度较低，表现出假塑性。它们具有乳化活性和结合某些金属的能力。
本研究中所研究的交替单胞菌科菌株产生的EPSs，其物理和化学性质与其他嗜盐和非嗜盐细菌产生的EPSs不同，这表明当今在高盐环境中遇到的微生物种类繁多，为生物技术应用提供了巨大的潜在资源。
我们优化了EPS的生产，并分析了一些最近描述的中度嗜盐细菌产生的新型生物聚合物。这些生物聚合物在化学和物理性质上与生物技术中已使用的其他生物聚合物不同，并为新的应用带来了希望，特别是对于西班牙交替单胞菌，它可能被证明是木寡糖的可行来源。
