Nagornev V A, Bobryshev Iu V, Ivanovskiĭ Iu V, Bogachev Iu V
Arkh Patol. 1991;53(3):23-9.
The study of the coronary arteries atherogenesis with the use of scanning and transmission electron microscopy was performed on the material of emergency necropsies of 45 patients who suffered the primary cardiovascular pathology. The transformation of macrophages into the foamy cells were studied in vitro with the use of peritoneal mouse macrophages and modified LDL labelled with colloidal gold as well as a method of phase-contrast cinemicrography. Two types of macrophages were distinguished ultrastructurally: scavenger-macrophages participating in the phagocytosis of lipoproteins and transforming into the foamy cells and secreting macrophages determining vascular wall homeostasis and ensuring the T-lymphocyte inclusion into the immune reactions and the contact with target cells. Balanced cell kinetics and cytokine secretion at early stages of atherosclerosis determine whether the lipid spot would regress or would transform into the atherosclerotic plaque.
利用扫描电子显微镜和透射电子显微镜对45例原发性心血管疾病患者的急诊尸检材料进行冠状动脉粥样硬化形成的研究。利用小鼠腹腔巨噬细胞、胶体金标记的修饰低密度脂蛋白以及相差电影显微镜技术在体外研究巨噬细胞向泡沫细胞的转化。超微结构上区分出两种巨噬细胞：参与脂蛋白吞噬并转化为泡沫细胞的清道夫巨噬细胞，以及决定血管壁内稳态并确保T淋巴细胞参与免疫反应并与靶细胞接触的分泌巨噬细胞。动脉粥样硬化早期阶段平衡的细胞动力学和细胞因子分泌决定了脂质斑点是否会消退或转化为动脉粥样硬化斑块。
