Lyle D B, Wang X H, Ayotte R D, Sheppard A R, Adey W R
Center for Devices and Radiological Health, Food and Drug Administration, Rockville, Maryland.
Bioelectromagnetics. 1991;12(3):145-56. doi: 10.1002/bem.2250120303.
Calcium-ion uptake by normal and leukemia lymphocytes increased during a 30-min exposure to a 13.6 Hz, sinusoidal magnetic field at 20 microT peak. The time-varying field was horizontal and parallel to a 16.5 microT component of the ambient static magnetic field. The uptake of 45Ca2+ increased 102% in a line of murine, cytotoxic T-lymphocytes (C57B1/6-derived CTLL-1), increased 126% in freshly-isolated spleen lymphocytes (C57B1/6 mice), and increased 75% in a line of lymphoma cells (C57B1/6-derived EL4). In contrast, there was no effect when the same field was applied for 30 min immediately before--as opposed to during--incorporation of calcium ions. When spleen lymphocytes were exposed during incubation with 45Ca2+ to a 60 Hz magnetic field at 20 microT peak, a small but statistically significant increase (37%) in uptake of the labeled ions occurred. These results indicate that weak, alternating magnetic fields might affect calcium-dependent functions of normal and leukemic lymphocytes.
在暴露于峰值为20微特斯拉的13.6赫兹正弦磁场30分钟期间，正常淋巴细胞和白血病淋巴细胞对钙离子的摄取增加。时变磁场是水平的，且与环境静磁场的16.5微特斯拉分量平行。在一组小鼠细胞毒性T淋巴细胞（C57B1/6来源的CTLL-1）中，45Ca2+的摄取增加了102%，在新鲜分离的脾淋巴细胞（C57B1/6小鼠）中增加了126%，在一组淋巴瘤细胞（C57B1/6来源的EL4）中增加了75%。相比之下，在钙离子掺入之前（而非掺入期间）立即施加相同磁场30分钟则没有效果。当脾淋巴细胞在与45Ca2+一起孵育期间暴露于峰值为20微特斯拉的60赫兹磁场时，标记离子的摄取出现了微小但具有统计学意义的增加（37%）。这些结果表明，弱交变磁场可能会影响正常和白血病淋巴细胞的钙依赖性功能。
