Prince P, Boulay M-E, Pagé N, Desmeules M, Boulet L-P
Centre de Recherche de l'Hôpital Laval, Institut de Cardiologie et de Pneumologie de l'Université Laval, Québec, Canada.
Int J Tuberc Lung Dis. 2008 Jul;12(7):813-9.
To evaluate the usefulness of fibrogenic cytokines and mediators in the analysis of induced sputum and determine if their levels correlated with previous decline in lung function in asbestosis and silicosis.
In a pilot study for the evaluation of 19 workers with asbestosis and 15 with silicosis, all workers had chart reviews and records of previous lung function tests. Fourteen healthy control subjects were also included in the study. All subjects attended the laboratory for a clinical evaluation, pulmonary function tests and induced sputum sampling. Differential cell counts were performed and the following mediators and cytokines were measured: matrix metalloproteinase 9 (MMP-9), tissue inhibitor of metalloproteinases 1 (TIMP-1), fibronectin, interleukin 1 beta (IL-1beta), IL-6, IL-8, IL-12, transforming growth factor beta (TGF-beta) and tumor necrosis factor-alpha (TNF-alpha).
Levels of IL-1beta were higher in the sputum of subjects with asbestosis and silicosis than in controls. Eosinophils, neutrophils and IL-1beta levels were significantly correlated with the rate of decline in pulmonary function.
The induced sputum levels of certain inflammatory cells and IL-1beta correlate with the decline in pulmonary function associated with asbestosis and silicosis. It remains to be established if these markers can help predict the clinical outcome of workers exposed to these mineral particles or fibers in a prospective study.
评估致纤维化细胞因子和介质在诱导痰分析中的作用，并确定其水平是否与石棉沉着病和矽肺患者先前的肺功能下降相关。
在一项初步研究中，对19名石棉沉着病工人和15名矽肺工人进行评估，所有工人均有病历回顾和先前肺功能测试记录。14名健康对照者也纳入研究。所有受试者到实验室进行临床评估、肺功能测试和诱导痰采样。进行细胞分类计数，并检测以下介质和细胞因子：基质金属蛋白酶9（MMP-9）、金属蛋白酶组织抑制剂1（TIMP-1）、纤连蛋白、白细胞介素1β（IL-1β）、IL-6、IL-8、IL-12、转化生长因子β（TGF-β）和肿瘤坏死因子-α（TNF-α）。
石棉沉着病和矽肺患者痰液中的IL-1β水平高于对照组。嗜酸性粒细胞、中性粒细胞和IL-1β水平与肺功能下降速率显著相关。
某些炎症细胞和IL-1β的诱导痰水平与石棉沉着病和矽肺相关的肺功能下降相关。在一项前瞻性研究中，这些标志物是否有助于预测接触这些矿物颗粒或纤维的工人的临床结局仍有待确定。
