Hotta Hiroki, Wang Qiang, Fukuda Mioko, Aizawa Shoichi, Umemura Tomonari, Sekizawa Kazutoshi, Tsunoda Kin-ichi
Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Japan.
Anal Sci. 2008 Jun;24(6):795-8. doi: 10.2116/analsci.24.795.
The use of electrospray ionization mass spectrometry (ESI-MS) in negative ion mode was investigated as a direct probe for identifying Al species in Al-accumulating hydrangea (Hydrangea macrophylla) samples. Cell sap solutions of hydrangea leaves were purified using Sephadex G-10 liquid chromatography and each fraction was analyzed using ESI-MS and ESI-MS/MS to identify Al species. In hydrangea leaves, a 1:1 Al-citrate complex was found as AlH(-1)cit (m/z 215), where H(3)cit denotes citric acid. This result is consistent with that of Ma et al. who used (27)Al-NMR.
研究了在负离子模式下使用电喷雾电离质谱（ESI-MS）作为直接探针，用于鉴定富铝绣球花（Hydrangea macrophylla）样品中的铝物种。绣球花叶的细胞液溶液通过葡聚糖凝胶G-10液相色谱进行纯化，并且使用ESI-MS和ESI-MS/MS对每个馏分进行分析以鉴定铝物种。在绣球花叶中，发现了一种1:1的铝-柠檬酸盐络合物，其形式为AlH(-1)cit（质荷比为215），其中H(3)cit表示柠檬酸。该结果与Ma等人使用（27）Al-NMR的结果一致。
