Technische Universität Braunschweig, Institute of Food Chemistry, Schleinitzstraße 20, 38106 Braunschweig, Germany.
University of Applied Sciences Erfurt, Erfurt Research Centre for Horticultural Crops, Kühnhäuser Straße 101, 99090 Erfurt, Germany.
J Agric Food Chem. 2022 Sep 21;70(37):11823-11831. doi: 10.1021/acs.jafc.2c04351. Epub 2022 Sep 11.
A wide range of secondary metabolites has been described for various species, including the sweet-tasting phenyldihydroisocoumarin phyllodulcin, which is found in the leaves of ssp. . This work aims at the development and validation of an analytical workflow for comprehensive semi-polar metabolite profiling using liquid chromatography coupled with electrospray ionization ion mobility quadrupole time-of-flight mass spectrometry (UPLC-ESI-IMS-QToF-MS) to complement existing analytical studies. The unsupervised analysis of this data set demonstrates the capability of this analytical workflow to distinguish different ssp. cultivars. In combination with supervised analysis, a list of metabolites responsible for the differentiation of the cultivars studied has been obtained. Suspect screening of phenyldihydroisocoumarins provides comprehensive information, which could help in the search for key enzymes related to the biosynthesis of phyllodulcin.
已描述了各种物种的广泛的次生代谢产物，包括存在于 ssp. 叶子中的具有甜味的苯并二氢异香豆素 Phyllodulcin。本工作旨在开发和验证一种使用液相色谱与电喷雾电离离子淌度四极杆飞行时间质谱（UPLC-ESI-IMS-QToF-MS）进行全面半极性代谢产物分析的分析工作流程，以补充现有的分析研究。对该数据集的无监督分析证明了该分析工作流程区分不同 ssp. 品种的能力。结合有监督分析，获得了一组负责区分所研究品种的代谢产物的列表。苯并二氢异香豆素的可疑筛选提供了全面的信息，这有助于寻找与 Phyllodulcin 生物合成相关的关键酶。
