Dev Shrabanti, Mizuguchi Hiroyuki, Das Asish K, Matsushita Chiyo, Maeyama Kazutaka, Umehara Hayato, Ohtoshi Takayuki, Kojima Jun, Nishida Kiyotaka, Takahashi Kunihiko, Fukui Hiroyuki
Department of Molecular Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
J Pharmacol Sci. 2008 Jun;107(2):159-66. doi: 10.1254/jphs.08028fp. Epub 2008 Jun 5.
It has been shown that probiotic bacteria are effective for the treatment of allergic diseases. As histamine plays a central role in allergic diseases, it is possible that probiotic bacteria affect the allergy-related histamine signaling. Here, we investigated the effect of Lac-B, a mixture of freeze-dried Bifidobacterium infantis and Bifidobacterium longum, on the allergy-related histamine signaling. In the nasal allergy model rats made by sensitization and provocation with toluene 2,4-diisocyanate (TDI) for 3 weeks, TDI provocation caused acute allergy-like behaviors along with significant up-regulation of histamine H(1) receptor (H1R) and histidine decarboxylase (HDC) mRNA expression, increased HDC activity, histamine content, and [(3)H]mepyramine binding activity in nasal mucosa. Prolonged treatment with Lac-B (40 mg/rat, p.o.) significantly suppressed both the allergy-like behaviors and all of the above mentioned factors involved in histamine signaling. Our findings indicate that oral administration of Lac-B showed significant anti-allergic effect through suppression of both H1R and HDC gene expression followed by decrease in H1R, HDC protein level, and histamine content. Suppression of histamine signaling may be a novel target of probiotics in preventing allergic diseases.
已表明益生菌对过敏性疾病的治疗有效。由于组胺在过敏性疾病中起核心作用，因此益生菌可能会影响与过敏相关的组胺信号传导。在此，我们研究了冻干婴儿双歧杆菌和长双歧杆菌的混合物Lac - B对与过敏相关的组胺信号传导的影响。在用2,4 - 二异氰酸甲苯酯（TDI）致敏和激发3周制成的鼻过敏模型大鼠中，TDI激发引起急性过敏样行为，同时组胺H(1)受体（H1R）和组氨酸脱羧酶（HDC）mRNA表达显著上调，鼻黏膜中HDC活性、组胺含量和[(3)H]美吡拉敏结合活性增加。用Lac - B（40 mg/大鼠，口服）长期治疗可显著抑制过敏样行为以及组胺信号传导中涉及的上述所有因素。我们的研究结果表明，口服Lac - B通过抑制H1R和HDC基因表达，随后降低H1R、HDC蛋白水平和组胺含量，显示出显著的抗过敏作用。抑制组胺信号传导可能是益生菌预防过敏性疾病的新靶点。
