Piirainen Laura, Kekkonen Riina A, Kajander Kajsa, Ahlroos Terhi, Tynkkynen Soile, Nevala Riikka, Korpela Riitta
Nutraceuticals Group, Institute of Biomedicine, Pharmacology, University of Helsinki, Helsinki, Finland.
Ann Nutr Metab. 2008;52(3):204-8. doi: 10.1159/000138124. Epub 2008 Jun 11.
The aim of this study was to compare a combination of Lactobacillus GG (LGG) and galacto-oligosaccharides (GOS) with LGG on its own, and their effects on the intestinal microbiota in school-aged children. The randomized, double-blinded, crossover study comprised 30 healthy children. There were two 3-week study periods with a 4-week wash-out period in between. The children ingested daily 65 ml of milk-based fruit juice containing either LGG alone (6.5 x 10(9) CFU) or LGG plus 2 g of GOS. Symptom diaries were filled during the study periods. Fecal samples were collected at the beginning and end of both study periods. At the end of both study periods, the amount of bifidobacteria was significantly greater after the ingestion of LGG + GOS compared with LGG alone (geometric mean 9.33 x 10(9) vs. 4.28 x 10(9) CFU/g, p < 0.001). No significant differences were seen in the amount of lactobacilli or LGG, nor did gastrointestinal symptoms, defecation frequency, consistency of stools or ease of defecation differ between the two study periods. Ingestion of LGG combined with 2 g of GOS increased the bifidobacteria more than LGG on its own and thus GOS clearly has a prebiotic effect in children. The children tolerated well a daily intake of 2 g of GOS.
本研究旨在比较鼠李糖乳杆菌GG（LGG）与低聚半乳糖（GOS）联合使用和单独使用LGG对学龄儿童肠道微生物群的影响。这项随机、双盲、交叉研究纳入了30名健康儿童。研究包括两个为期3周的阶段，中间有4周的洗脱期。孩子们每天摄入65毫升含LGG（6.5×10⁹CFU）或LGG加2克GOS的牛奶基果汁。在研究期间填写症状日记。在两个研究阶段开始和结束时收集粪便样本。在两个研究阶段结束时，与单独摄入LGG相比，摄入LGG + GOS后双歧杆菌数量显著增加（几何平均数9.33×10⁹对4.28×10⁹CFU/g，p < 0.001）。两个研究阶段之间，乳酸菌或LGG数量以及胃肠道症状、排便频率、粪便稠度或排便难易程度均无显著差异。摄入LGG与2克GOS联合使用比单独摄入LGG更能增加双歧杆菌数量，因此GOS对儿童显然具有益生元作用。孩子们对每天摄入2克GOS耐受性良好。
