Nascentes Gabriel Antonio Nogueira, Meira Wendell Sérgio Ferreira, Lages-Silva Eliane, Ramírez Luis Eduardo
Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Uberaba, MG.
Rev Soc Bras Med Trop. 2008 Mar-Apr;41(2):152-7. doi: 10.1590/s0037-86822008000200004.
This study evaluated the possibility of inoculation and reinoculation with a trypanosomatid isolated from bats that is morphologically, biologically and molecularly similar to Trypanosoma cruzi, to protect against infection by virulent strains. Non-isogenic mice were divided into 24 groups that received from zero to three inoculations of Trypanosoma cruzi-like strain RM1, in the presence or absence of Freunds adjuvant, and were challenged with the VIC or JG strains of Trypanosoma cruzi. Parasitemia and survival were monitored and animals were sacrificed for histopathological analysis. Animals immunized with Trypanosoma cruzi-like strain RM1 presented decreased parasitemia, independently of the number of inoculations or the presence of adjuvant. In spite of this reduction, these animals did not present any protection against histopathological lesions. Severe eosinophilic infiltrate was observed and was correlated with the number of inoculations of Trypanosoma cruzi-like strain RM1. These findings suggest that prior inoculation with this strain did not protect against infection but, rather, aggravated the tissue inflammatory process.
本研究评估了用从蝙蝠中分离出的一种锥虫进行接种和再接种的可能性，该锥虫在形态、生物学和分子水平上与克氏锥虫相似，以预防强毒株的感染。非同基因小鼠被分为24组，在有或没有弗氏佐剂的情况下，接受0至3次克氏锥虫样菌株RM1的接种，并用克氏锥虫的VIC或JG菌株进行攻击。监测寄生虫血症和存活率，并对动物进行处死以进行组织病理学分析。用克氏锥虫样菌株RM1免疫的动物寄生虫血症降低，这与接种次数或佐剂的存在无关。尽管有这种降低，但这些动物对组织病理学损伤没有任何保护作用。观察到严重的嗜酸性粒细胞浸润，且与克氏锥虫样菌株RM1的接种次数相关。这些发现表明，预先接种该菌株并不能预防感染，反而会加重组织炎症过程。
