Kim Sung Hun, Kim Shukho, Chun Sung Guen, Park Mi-Sun, Park Jeong Hyun, Lee Bok-Kwon
Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul 122-701, Republic of Korea.
J Microbiol. 2008 Apr;46(2):209-13. doi: 10.1007/s12275-007-0197-1. Epub 2008 Jun 11.
We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.
我们在2002年分析了66株肠炎沙门氏菌分离株。其中30株分离自腹泻的人类患者，36株分离自鸡。在人类和鸡的分离株中总共鉴定出10种噬菌体类型（PT）。PT1和PT21是人类（分别为20%和13%）和鸡（分别为17%和47%）分离株中的主要噬菌体类型。通过脉冲场凝胶电泳（PFGE）产生了12种脉冲型，并分为两个主要组。大多数PFGE类型被归类为聚类组1。聚类组1中的18株鸡分离株与其他22株人类分离株显示出高度的遗传关联（>95%）。此外，聚类组2中的6株鸡分离株与其他7株人类分离株显示出相当高的遗传关联（>95%）。在人类和鸡中，与A5-PT21（11株分离株）、A2-PT1（7株分离株）和B1-PT4（6株分离株）的遗传关联水平最高。脉冲场凝胶电泳（PFGE）和噬菌体分型提供了确凿证据，表明人类沙门氏菌感染归因于食用受污染的鸡肉。
