Luo Zhongli, Zhao Xiaojun, Zhang Shuguang
West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu, Sichuan 610065, China.
Macromol Biosci. 2008 Aug 11;8(8):785-91. doi: 10.1002/mabi.200800003.
Self-assembling peptide nanofiber scaffolds are an excellent material for applications such as tissue repair, tissue regeneration, instant stopping of bleeding, and slow drug release. We report a new self-assembling peptide D-EAK16 consisting purely of D-amino acids. D-EAK16 and L-EAK16 display mirror-image CD spectra at 20 degrees C. Like L-EAK16, D-EAK16 self-assembles into nanofibers, thus demonstrating that chiral self-assembling peptide nanofiber scaffolds can be made from both L- and D-amino acids. We also show that D-peptide nanofibers are resistant to natural proteases and may thus be useful in biotechnology, nanobiotechnology, tissue repair and tissue regeneration as well as other medical applications.
自组装肽纳米纤维支架是用于组织修复、组织再生、即时止血和缓释药物等应用的优良材料。我们报道了一种全新的仅由D-氨基酸组成的自组装肽D-EAK16。D-EAK16和L-EAK16在20摄氏度时呈现镜像圆二色光谱。与L-EAK16一样，D-EAK16自组装成纳米纤维，从而证明手性自组装肽纳米纤维支架可以由L-氨基酸和D-氨基酸制成。我们还表明，D-肽纳米纤维对天然蛋白酶具有抗性，因此可能在生物技术、纳米生物技术、组织修复和组织再生以及其他医学应用中有用。
