Chen Shuo, Zhou Ao, He Bin, Zhao Weikang, Chen Xiaojun, Jiang Dianming
Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Int J Mol Med. 2017 Sep;40(3):679-688. doi: 10.3892/ijmm.2017.3056. Epub 2017 Jul 5.
Self-assembling peptide (SAP) nanofiber hydrogel scaffolds have become increasingly important in tissue engineering due to their outstanding bioactivity and biodegradability. However, there is an initial concern on their long-term clinical use, since SAPs made of L-form amino acid sequences are sensitive to enzymatic degradation. In this study, we present a designer SAP, D-RADA16, made of all D-amino acid. We investigated the nanofiber morphology of D-RADA16, its potential for the culture of bone marrow-derived mesenchymal stem cells (BMSCs), and the proteolytic resistance of the biomaterial. The results revealed that D-RADA16 exhibited stable β-sheets and formed interwoven nanofiber scaffolds in water. D-RADA16 and L-RADA16 hydrogel scaffolds were both found to promote the proliferation and migration of rat BMSCs in the 3D cell culture microenvironment. Furthermore, the D-RADA16 scaffolds exhibited a higher proteolytic resistance against proteinase K than the L-RADA16 scaffolds. These observations indicate that D-RADA16 hydrogel scaffolds have excellent bioactivity, biocompatibility and biostability, and thus may serve as promising candidates for long-term application in vivo.
自组装肽（SAP）纳米纤维水凝胶支架因其出色的生物活性和生物可降解性，在组织工程中变得越来越重要。然而，人们最初对其长期临床应用存在担忧，因为由L型氨基酸序列制成的SAP对酶降解敏感。在本研究中，我们展示了一种由所有D型氨基酸制成的定制SAP，即D-RADA16。我们研究了D-RADA16的纳米纤维形态、其用于培养骨髓间充质干细胞（BMSC）的潜力以及该生物材料的抗蛋白酶解性。结果显示，D-RADA16呈现出稳定的β折叠片层，并在水中形成交织的纳米纤维支架。在三维细胞培养微环境中，D-RADA16和L-RADA16水凝胶支架均被发现可促进大鼠BMSC的增殖和迁移。此外，D-RADA16支架对蛋白酶K的抗蛋白酶解性高于L-RADA16支架。这些观察结果表明，D-RADA16水凝胶支架具有出色的生物活性、生物相容性和生物稳定性，因此可能成为体内长期应用的有前途的候选材料。
