Department of Chemical Engineering, Kyushu University 36, Fukuoka, 812 Japan.
Biotechnol Bioeng. 1982 Feb;24(2):347-54. doi: 10.1002/bit.260240208.
As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis-Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters V(m) and K(m) vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 x 10(4) and 3 x 10(6).
当淀粉通过糖化酶水解时，葡萄糖单元从淀粉链的非还原端逐步去除，在降解过程中，可用底物分子的数量基本保持不变。鉴于这一方面，本文提出了一种用于可溶性淀粉水解的简单实用的动力学表达式，它由一个带有产物抑制的改良米氏-门坦形式组成。假定动力学参数 V(m)和 K(m)的值从淀粉的值线性变化到麦芽糖的值。通过对两种平均分子量分别为 3 x 10(4)和 3 x 10(6)的可溶性淀粉水解的实验结果进行模拟，验证了该动力学表达式的适用性。
