Biochemical Engineering Research Centre, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India.
Biotechnol Bioeng. 1982 Apr;24(4):805-15. doi: 10.1002/bit.260240405.
In earlier communications general analyses of rapid ethanol fermentation by Saccharomyces cerevisiae immobilized on inert supports were described. In this article physiology of growth and metabolism (parameters like rates of CO(2) evolution and O(2) uptake, respiratory quotient, and generation time) of Saccharomyces cerevisiae immobilized on different supports are reported. Values of the ratio of specific oxygen uptake rate for immobilized cells to free cells have been found to be 0.732, 0.781 and 0.785 for carrier A, carrier B, and covalently crosslinked controlled pore glass (CPG, specific surface area of 439 m(2) g(-1)), respectively. Rates of specific CO(2) evolution for immobilized cells to free cells for these supports are 0.784, 0.822, and 0.783, respectively. Marked reduction in generation time of Saccharomyces cerevisiae on all the supports has been observed. No change in size (4.8-5 microm) and specific growth rate (micro(m) = 0.275 h(-1)) of cells leaving the reactor has been observed.
在早期的通讯中，描述了固定在惰性载体上的酿酒酵母快速乙醇发酵的综合分析。本文报道了不同载体固定化的酿酒酵母的生长和代谢生理学（如二氧化碳释放和氧气摄取速率、呼吸商和世代时间等参数）。发现载体 A、载体 B 和共价交联的控制孔玻璃（CPG，比表面积为 439 m(2) g(-1))上固定化细胞比游离细胞的比耗氧速率比值分别为 0.732、0.781 和 0.785。对于这些载体，固定化细胞与游离细胞的比 CO(2) 释放速率分别为 0.784、0.822 和 0.783。在所有载体上，酿酒酵母的世代时间都明显缩短。离开反应器的细胞的大小（4.8-5 微米）和比生长速率（micro(m) = 0.275 h(-1))没有变化。
