Willaert R G, Baron G V
Department of Chemical Engineering, Vrije Universiteit Brussel, Belgium.
Appl Microbiol Biotechnol. 1995 Jan;42(5):664-70. doi: 10.1007/BF00171941.
Membrane mass spectrometry (MMS) with reduced sample withdrawal has been used to investigate the metabolic activity of yeast cells immobilised in porous glass. An adapted MS membrane inlet reactor with a polyethylene terephthalate barrier membrane has been constructed for this purpose. In a first experiment, the mass transport of O2 in a porous glass disc under well-defined experimental conditions has been studied by determining the apparent effective diffusion coefficient. The behaviour of immobilised Saccharomyces cerevisiae has been monitored by the MMS measurement of O2 and CO2 after applying a step in glucose concentration. Free-cell kinetic parameters were used in a dynamic reaction-diffusion model to simulate the O2 consumption curve. The theoretical and experimental curve showed comparable behaviour, which means that the immobilisation of yeast cells in porous glass has no substantial effect on its growth kinetics.
减少样品抽取量的膜质谱法（MMS）已用于研究固定在多孔玻璃中的酵母细胞的代谢活性。为此构建了一种带有聚对苯二甲酸乙二酯阻挡膜的改良型MS膜进样反应器。在第一个实验中，通过测定表观有效扩散系数，研究了在明确的实验条件下氧气在多孔玻璃盘中的质量传输。在葡萄糖浓度阶跃变化后，通过对氧气和二氧化碳的MMS测量来监测固定化酿酒酵母的行为。将游离细胞动力学参数用于动态反应扩散模型以模拟氧气消耗曲线。理论曲线和实验曲线表现出相似的行为，这意味着酵母细胞在多孔玻璃中的固定化对其生长动力学没有实质性影响。
