基于聚合酶链反应（PCR）对乳牙和恒牙牙髓感染相关特定病原体的鉴定。
PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth.
作者信息
Cogulu Dilsah, Uzel Atac, Oncag Ozant, Eronat Cemal
机构信息
Department of Pedodontics, Ege University, School of Dentistry, Bornova-Izmir, Turkey.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Sep;106(3):443-9. doi: 10.1016/j.tripleo.2008.03.004. Epub 2008 Jun 11.
OBJECTIVE
The aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using PCR method and to determine the association of these organisms with clinical symptoms.
STUDY DESIGN
A total of 145 children, 5 to 13 years old, were involved in this study. The presence of selected pathogens (Actinomyces israelii, Candida albicans, Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus intermedius, Treponema denticola, Parvimonas micra, Tannerella forsythensis, Enterococcus faecium, Prevotella melaninogenica) in infected root canals was studied using PCR.
RESULTS
T. denticola (P = .012, .02) and E. faecalis (P = .012, .04) were highly associated with periapical radiolucency and previous pain, while P. gingivalis was associated with tenderness to percussion in both deciduous and permanent teeth (P = .01, .015).
CONCLUSION
The results of the present study confirm that certain species of microorganisms are associated with clinical signs and symptoms of endodontic disease in both deciduous and permanent teeth.
目的
本研究旨在通过聚合酶链反应（PCR）方法评估乳牙和恒牙根管样本中所选病原体的存在情况，并确定这些微生物与临床症状之间的关联。
研究设计
本研究共纳入145名5至13岁的儿童。采用PCR技术研究感染根管中所选病原体（以色列放线菌、白色念珠菌、粪肠球菌、具核梭杆菌、牙髓卟啉单胞菌、牙龈卟啉单胞菌、中间普氏菌、中间链球菌、齿垢密螺旋体、微小单胞菌、福赛坦氏菌、屎肠球菌、产黑色素普氏菌）的存在情况。
结果
齿垢密螺旋体（P = 0.012，0.02）和粪肠球菌（P = 0.012，0.04）与根尖周透射区和既往疼痛高度相关，而牙龈卟啉单胞菌与乳牙和恒牙的叩诊压痛相关（P = 0.01，0.015）。
结论
本研究结果证实，某些微生物种类与乳牙和恒牙牙髓病的临床体征和症状相关。
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