Kosta Vana, Kojundzić Sanja Lovrić, Sapunar Liana Cambj, Sapunar Damir
Department of Anatomy, Histology and Embryology, University of Split Medical School, Split, Croatia.
Eur J Pain. 2009 Mar;13(3):243-8. doi: 10.1016/j.ejpain.2008.04.012. Epub 2008 Jun 10.
One of the unresolved questions in neuropathic pain research is whether we can prevent or reverse mechanical hyperalgesia by rhizotomy or ganglionectomy. However, one of the obstacles in answering that question is lack of a standardized surgical procedure used in experimental ganglionectomy. We tested the hypothesis that laminectomy performed during ganglionectomy induces lumbar column deformity. We further examined whether spinal deformity is a source of pain-related behavior. Five conditions were studied. Fifth and sixth lumbar (L5 and L6) ganglionectomy were performed in rats using either minimal or extensive laminectomy technique. Two other groups had minimal and extensive laminectomy without ganglionectomies. A final control group had no surgery. Sensory responsiveness of the plantar aspect of the hind paw was repeatedly tested, and a plain radiograph in anteroposterior projection was made to assess the extent of deformity by measurement of deformity angles. Hyperalgesia resulted in groups with extensive laminectomy regardless of performance or absence of ganglionectomy, while in groups with minimal laminectomy there was no increase in pain-related behavior. Lateral deformity of the spine was observed in rats with or without ganglionectomy, confirming that laminectomy can produce deformity. The extent of deformity was more pronounced in rats exposed to the extensive laminectomy. Our results indicate that laminectomy can produce spine deformity and that there is a direct relationship between the extent of laminectomy and the development of mechanical hypersensitivity. The data presented suggest that there is a need for standardization of laminectomy procedure in rat experimental pain models.
神经病理性疼痛研究中尚未解决的问题之一是，我们是否能够通过神经根切断术或神经节切除术预防或逆转机械性痛觉过敏。然而，回答这个问题的障碍之一是缺乏实验性神经节切除术中使用的标准化手术程序。我们检验了这样一个假设，即神经节切除术中进行的椎板切除术会导致腰椎畸形。我们进一步研究了脊柱畸形是否是疼痛相关行为的一个来源。我们研究了五种情况。使用最小限度或广泛椎板切除技术对大鼠进行第五和第六腰椎（L5和L6）神经节切除术。另外两组分别进行了最小限度和广泛椎板切除术，但未进行神经节切除术。最后一个对照组未进行手术。反复测试后爪足底方面的感觉反应性，并拍摄前后位平片，通过测量畸形角度来评估畸形程度。无论是否进行神经节切除术，广泛椎板切除术的组均出现痛觉过敏，而最小限度椎板切除术的组中与疼痛相关的行为没有增加。无论是否进行神经节切除术，大鼠均观察到脊柱侧方畸形，证实椎板切除术可导致畸形。广泛椎板切除术的大鼠畸形程度更明显。我们的结果表明，椎板切除术可导致脊柱畸形，并且椎板切除范围与机械性超敏反应的发生之间存在直接关系。所呈现的数据表明，在大鼠实验性疼痛模型中需要对椎板切除手术进行标准化。
