Gong C S, Claypool T A, McCracken L D, Maun C M, Ueng P P, Tsao G T
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Biotechnol Bioeng. 1983 Jan;25(1):85-102. doi: 10.1002/bit.260250108.
The utilization and conversion of D-xylose, D-xylulose, L-arabinose, and xylitol by yeast strains have been investigated with the following results: (1) The majority of yeasts tested utilize D-xylose and produce polyols, ethanol, and organic acids. The type and amount of products formed varies with the yeast strains used. The most commonly detected product is xylitol. (2)The majority of yeasts tested utilize D-xylulose aerobically and fermentatively to produce ethanol, xylitol, D-arabitol, and organic acids. The type and amount of products varies depending upon the yeast strains used. (3) Xylitol is a poor carbon and energy source for most yeasts tested. Some yeast strains produce small amounts of ethanol from xylitol. (4) Most yeast strains utilize L-arabinose, and L-arabitol is the common product. Small amounts of ethanol are also produced by some yeast strains. (5) Of the four substrates examined, D-xylulose was the perferred substrate, followed by D-xylose, L-arabinose, and xylitol. (6) Mutant yeast strains that exhibit different metabolic product patterns can be induced and isolated from Candida sp. Saccharomyces cerevisiae, and other yeasts. These mutant strains can be used for ethanol production from D-xylose as well as for the study of metabolic regulation of pentose utilization in yeasts.
对酵母菌株利用和转化D-木糖、D-木酮糖、L-阿拉伯糖和木糖醇的情况进行了研究,结果如下:(1) 大多数受试酵母利用D-木糖并产生多元醇、乙醇和有机酸。所形成产物的类型和数量因所用酵母菌株而异。最常检测到的产物是木糖醇。(2) 大多数受试酵母在需氧和发酵条件下利用D-木酮糖,产生乙醇、木糖醇、D-阿拉伯糖醇和有机酸。产物的类型和数量取决于所用酵母菌株。(3) 木糖醇对大多数受试酵母来说是一种较差的碳源和能源。一些酵母菌株能从木糖醇中产生少量乙醇。(4) 大多数酵母菌株利用L-阿拉伯糖,常见产物是L-阿拉伯糖醇。一些酵母菌株也产生少量乙醇。(5) 在所研究的四种底物中,D-木酮糖是首选底物,其次是D-木糖、L-阿拉伯糖和木糖醇。(6) 可以从假丝酵母属、酿酒酵母和其他酵母中诱导并分离出表现出不同代谢产物模式的突变酵母菌株。这些突变菌株可用于从D-木糖生产乙醇以及研究酵母中戊糖利用的代谢调控。