Ooshima H, Harano Y
Department of Applied Chemistry, Faculty of Engineering, Osaka City University, Osaka 558, Japan.
Biotechnol Bioeng. 1983 Jan;25(1):143-55. doi: 10.1002/bit.260250112.
Taking the hydrolysis of sucrose by invertase immobilized on anion-exchange resin as an example, the effects of mass-transfer resistance on the apparent stability of immobilized enzyme (IME) and the optimal policy for an IME reaction in a fixed-bed reactor have been studied theoretically and experimentally. The following results were obtained: (1) The effect of mass-transfer resistance on the effective deactivation rate of IME is summarized in two parameters concerning the intraparticle diffusion alpha(p) and the interparticle alpha(f). (2) At a constant processed amount of raw materials, there exists an optimal flow rate of reaction fluid to enhance the reactor performance while the mass-transfer resistance shifts the optimal point. (3) The intrinsic deactivation rate of IME has been estimated from the relationship between the fractional conversion at the reactor outlet and the operation time.
以固定在阴离子交换树脂上的转化酶催化蔗糖水解为例，从理论和实验两方面研究了传质阻力对固定化酶（IME）表观稳定性的影响以及固定床反应器中IME反应的最优策略。得到以下结果：（1）传质阻力对IME有效失活速率的影响归纳为两个参数，即颗粒内扩散α(p)和颗粒间α(f)。（2）在原料处理量恒定的情况下，存在一个使反应器性能增强的最优反应流体流速，而传质阻力会使最优点发生偏移。（3）根据反应器出口处的转化率与运行时间之间的关系估算了IME的固有失活速率。
