Moloney A, Coughlan M P
Department of Biochemistry, University College, Galway, Ireland.
Biotechnol Bioeng. 1983 Jan;25(1):271-80. doi: 10.1002/bit.260250120.
The sorption characteristics of the cellulase system of Talaromyces emersonii on various cellulosic substrates were examined. Analysis of reaction mixture supernatants by electrophoresis and enzyme assay showed that all components of the cellulase system were rapidly adsorbed by cellulose and then gradually returned to the liquid phase as the hydrolysis of the substrate progressed. The extent of adsorption in the rapid phase was influenced by pH, temperature, the nature of the substrate, and its concentration.
考察了埃默森篮状菌纤维素酶系统在各种纤维素底物上的吸附特性。通过电泳和酶活性测定对反应混合物上清液进行分析,结果表明,纤维素酶系统的所有组分都能迅速被纤维素吸附,然后随着底物水解的进行逐渐回到液相。快速吸附阶段的吸附程度受pH、温度、底物性质及其浓度的影响。